BIO409/509 Cell and Molecular Biology
SECOND Methods paper assignment due Wed., 4/20
(you don’t do this assignment if you
are in the 4H STEM Ambassador
Program)
Assignment: Choose a SECOND method in Molecular Biology or Cell Biology
and write 2-3 page paper about the following:
1)Describe the steps done in the method
2)Explain the “highlight” steps: What are the key “tricks” in the method.
3)Describe two different applications of the method to help demonstrate
how the method is valuable.
On an extra page, include a bibliography with any resources you used:
Our textbook, web sites, personal communications, journal articles, etc.
BIO409/509 Cell and Molecular Biology
We will skip Chapter 7 DNA Replication
and never cover it. Finish Chapter 8 RNA
Synthesis on on to Chapter 9 Protein
Synthesis. That’s the end of Part II. We’ll
start talking about Chapter 10 The
Nucleus this week.
RNA Synthesis andProcessing
8
Figure 8.31 Histone acetylation (Part2)
Figure 8.37 DNA methylation
Figure 8.38 Maintenance ofmethylation patterns
Each step in gene expression has the potential for being regulated if it is the rate
limiting step.
Regulation of Protein Function
•Cells can regulate the amounts and theactivities of their proteins.
•Three mechanisms:
•Regulation by small molecules
•Phosphorylation and other modifications
•Protein-protein interactions
Figure 8.44 Processing of eukaryoticmessenger RNAs (Part 1)
Figure 8.47 Splicing of pre-mRNA
Figure 8.48 Assembly of thespliceosome (Part 2)
Figure 8.52 Alternative splicing inDrosophila sex determination
Figure 8.55 mRNA degradation
Protein Synthesis,Processing, and Regulation
9
9 Protein Synthesis, Processing, andRegulation
•Translation of mRNA
•Protein Folding and Processing
•Regulation of Protein Function
•Protein Degradation
Figure 9.9 Overview of translation
Figure 9.7 Prokaryotic and eukaryoticmRNAs
Figure 9.8 Signals for translationinitiation
Table 9.1 Translation Factors
Figure 9.11 Initiation of translation ineukaryotic cells (Part 1)
Figure 9.17 Translational regulation offerritin
Figure 9.22 Regulation of eIF4E
Translation of mRNA
•RNA interference is mediated by:
•Small interfering RNAs (siRNAs)—produced from double-stranded RNAs bythe nuclease Dicer.
•MicroRNAs (miRNAs)—transcribed byRNA polymerase II, then cleaved bynucleases Drosha and Dicer.
Figure 9.13 Elongation stage oftranslation (Part 1)
Figure 9.13 Elongation stage oftranslation (Part 3)